Carbamyl Phosphate Binding to Aspartate Transcarbamylase
نویسندگان
چکیده
منابع مشابه
Carbamyl phosphate-aspartate transcarbamylase activity in tumors.
The enzyme carbamyl phosphate-aspartate transcarbamylase catalyzes the transfer of the carbamyl group from carbamyl phosphate (CP) to L-aspartate ($). Preliminary experiments with a series of six relatively advanced azo dye-induced hepatomas indicated that hepatoma tissue had a higher CP-aspartate transcarbamylase activity than did liver from normal animals. Experiments are reported here in whi...
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An increase in CP-asp. 1 transcarbamylase activity in experimentally produced rat hepatoma was reported in a previous communication (5). The question whether the increased activity is characteristic of neoplasia or is simply a reflection of the metabolic changes taking place in rapidly growing tissue presented itself. Since liver tissue has the capacity to grow relatively rapidly during the pro...
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The enzyme carbamyl phosphate-ornithine transcarbamylase, which catalyzes the synthesis of citrulline from ornithine plus carbamyl phosphate, has been partially purified from rat liver by means of heat treatment and ethanol fractionation (1). Krebs et al. (2) were able to demonstrate the arsenolysis of citrulline in a number of tissues and, by using sheep liver mitochondria, were able to purify...
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Leishmania donovani is a protozoal pathogen that belongs to the kinetoplastida order. Unlike in other eucaryotic systems, the first three enzymes of the de novo pyrimidine biosynthetic pathway are not components of a multifunctional protein system. The three enzyme activities in the crude extract were separated on a Sephacryl S-200 column. Aspartate carbamoyltransferase (EC 2.1.3.2) has been pu...
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Aspartate transcarbamylase from a Pseudomonas fluerescens has been purified to homogeneity. The enzyme has a molecular weight of 360,000. It is composed of 2 apparently identical subunits with molecular weights of 180,000. Activity can be recovered from the enzyme denatured with mercaptoethanol and sodium dodecyl sulfate when the dodecyl sulfate is dialyzed away. The renatured enzyme has the mo...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1973
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)43671-5